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Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
  • 작성일2019-05-14
  • 최종수정일2019-05-27
  • 담당부서연구기획과
  • 연락처043-719-8033
  • 949
Oncotarget, 2018, 9(45), 27656─27666, DOI: https://doi.org/10.18632/oncotarget.24441

Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer

Jong-Young Lee, Pildu Jeong; Xuan-Mei Piao; Ye-Hwan Kim; Jihye Kim; Sathiyamoorthy Subramaniyam; Young Joon Byun; Ho Won Kang; Sung Phil Seo; Jayoung Kim; Jung Min Kim; Eun Sang Yoo; Isaac Y. Kim; Sung-Kwon Moon; Yung Hyun Choi; Wun-Jae Kim

Abstract

    Background: Differentially expressed genes and their post-tranional regulator-microRNAs (miRNAs), are potential keys to pioneering cancer diagnosis and treatment. The aim of this study was to investigate how the miRNA-mRNA interactions might affect the tumorigenesis of bladder cancer (BC) and to identify specific miRNA and mRNA genetic markers in the two BC types: non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC).
    Results: We identified 227 genes that interacted with 54 miRNAs in NMIBC, and 14 genes that interacted with 10 miRNAs in MIBC. Based on this data, we found extracellular matrix-related genes are highly enriched in NMIBC while genes related to core nuclear division are highly enriched in MIBC. Furthermore, using a tranional regulatory element database, we found indirect regulatory tranion factors (TFs) for enriched genes could regulate tumorigenesis with or without miRNAs.
    Materials and methods: Tissue samples from 234 patients histologically diagnosed with BC and 83 individuals without BC were analyzed using microarray and next-generation sequencing technology, and we used different cut-offs to identify differentially expressed mRNAs and miRNAs in NMIBC and MIBC. The selected mRNAs and miRNAs were paired using validated target datasets and according to inverse expression relationships. MiRNA interacted genes were compared with the TF-regulating genes in BC. Meanwhile, pathway enrichment analysis was performed to identify the functions of selected miRNAs and genes.
    Conclusions: Identification of differential gene expression in specific tumor types could facilitate development of cancer diagnosis and aid in the early detection of BC.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2018-보건의료생물자원종합관리) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2018-보건의료생물자원종합관리) by Research of Korea Centers for Disease Control and Prevention.


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