본문으로 바로가기 주메뉴 바로가기

사용자별 맞춤메뉴

자주찾는 메뉴

추가하기
닫기

연구성과

contents area

detail content area

Genetic and metabolic comparison of orthotopic and heteroopic patient-dervied pancreatic-cancer xenografts to the original patient tumors
  • 작성일2019-05-14
  • 최종수정일2019-05-27
  • 담당부서연구기획과
  • 연락처043-719-8033
  • 998
oncotarget, 2018, 9(8), 7867─7881, DOI: https://doi.org/10.18632/oncotarget.23567

Genetic and metabolic comparison of orthotopic and heteroopic patient-dervied pancreatic-cancer xenografts to the original patient tumors

Eunsung Jun, Seung-Mo Hong; Hyun Ju Yoo; Moon-Bo Kim; Ji Sun Won; Soyeon An; In Kyong Shim; Suhwan Chang; Robert M. Hoffman; Song Cheol Kim

Abstract

    Tumors from 25 patients with pancreatic cancer were used to establish two patient-derived xenograft (PDX) models: orthotopic PDX (PDOX) and heterotopic (subcutaneous) PDX (PDHX). We compared gene expression by immunohistochemistry, single-nucleotide polymorphism (SNP), DNA methylation, and metabolite levels. The 4 cases, of the total of 13 in which simultaneous PDHX & PDOX models were established, were randomly selected. The molecular-genetic characteristics of the patient’s tumor were well maintained in the two PDX models. SNP analysis demonstrated that both groups were more than 90% identical to the original patient’s tumor, and there was little difference between the two models. DNA methylation of most genes was similar among the two models and the original patients tumor, but some gene sets were hypermethylated the in PDOX model and hypomethylated in the PDHX model. Most of the metabolites had a similar pattern to those of the original patient tumor in both PDX tumor models, but some metabolites were more prominent in the PDOX and PDHX models. This is the first simultaneous molecular-genetic and metabolite comparison of patient tumors and their tumors established in PDOX and PDHX models. The results indicate high fidelity of these critical properties of the patient tumors in the two models.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2018-보건의료생물자원종합관리) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2018-보건의료생물자원종합관리) by Research of Korea Centers for Disease Control and Prevention.


본 공공저작물은 공공누리  출처표시+상업적이용금지+변경금지 조건에 따라 이용할 수 있습니다 본 공공저작물은 공공누리 "출처표시+상업적이용금지+변경금지" 조건에 따라 이용할 수 있습니다.
TOP