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Characterization of Specific IgA Response to Antigenic Determinants of Helicobacter pylori Urease Encoded by ureA and ureB in Children
  • 작성일2019-05-14
  • 최종수정일2019-05-27
  • 담당부서연구기획과
  • 연락처043-719-8033
  • 948
Journal of Bacteriology and Virology , 2018, 48(1), 14─22, DOI: https://doi.org/10.4167/jbv.2018.48.1.14

Characterization of Specific IgA Response to Antigenic Determinants of Helicobacter pylori Urease Encoded by ureA and ureB in Children

Min-Kyoung Shin, Jin-Su Ju; Soon-Wook Kwon; Dong-Hae Lee; Jong-Hun Ha; Jin-Sik Park; Dae Hyun Song; Myung Hwan Jung; Hyung-Lyun Kang; Seung-Chul Baik; Ji Sook Park; Hee-Shang Youn; Myung Je Cho; Ji-Hyun Seo; Woo-Kon Lee

Abstract

    Helicobacter pylori (H. pylori ), a causative agent of chronic gastritis and gastric cancer, has several virulent factors for own survival and progression toward gastric diseases in human stomach. Of those, H. pylori produces mainly urease (10~15% total protein weight) that neutralize the gastric acid for survival. Here, we identified the antigenic epitope of urease and then developed an ELISA using the antigen including the epitope of urease. We identified the antigenic epitope of urease that induces IgA antibodies in human using truncated mutants. Eight kinds of seriallytruncated mutant of UreA and UreB were prepared and subjected to immunoblot using pooled sera of patients with gastric disorders. UreBEnd protein containing UreB epitope was produced and investigated its diagnostic value via ELISA in children. As a result, mutants having last 24 amino acid residues of UreB carboxyl terminus deleted did not show IgA-reactive band. The clones that contained the downstream of 448th amino acid in UreB showed IgA-reactive band. The serodiagnostic value of the UreBEnd recombinant protein including identified epitope was confirmed via IgA ELISA and shown to have 97% sensitivity and 100% specificity. These results demonstrated that carboxyl terminal region of UreB carries an antigenic epitope for IgA response in human. It may be useful for detecting H. pylori infection with improved test accuracy and minimum use of endoscopy.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2018-보건의료생물자원종합관리) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2018-보건의료생물자원종합관리) by Research of Korea Centers for Disease Control and Prevention.


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