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Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring
  • 작성일2019-05-09
  • 최종수정일2019-05-09
  • 담당부서연구기획과
  • 연락처043-719-8033
  • 537
Lab on a Chip, 2018, 18(9), 1320─1329, DOI: https://doi.org/10.1039/c8lc00165k

Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring

Chi-Ju Kim, Juhee Park; Vijaya Sunkara; Tae-Hyeong Kim; Yongjin Lee; Kyusang Lee; Mi-Hyun Kim ; Yoon-Kyoung Cho

Abstract

    The potential utility of circulating tumour DNA (ctDNA) in patient blood for cancer diagnostics and realtime monitoring of disease progression is highly recognized. However, the lack of automated and efficient methods for cell-free DNA (cfDNA) isolation from peripheral blood has remained a challenge for broader acceptance of liquid biopsy in general clinical settings. Here, we demonstrate a lab-on-a-disc system equipped with newly developed, electromagnetically actuated, and reversible diaphragm valves that allows fully automated and rapid (<30 min) isolation of cfDNA from whole blood (>3 ml) to achieve high detection sensitivity by minimizing the degradation of fragile ctDNA as well as contamination of wild-type DNA from abundant blood cells. As a proof of concept study, we used the lab-on-a-disc to isolate cfDNA from patients with non-small cell lung cancer and successfully detected epidermal growth factor receptor gene mutations (L858R, T790M) during targeted drug therapy. The proposed lab-on-a-disc enables a fully automated, rapid, and point-of-care cfDNA enrichment starting from whole blood to facilitate the wide use of liquid biopsy in routine clinical practice.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2018-보건의료생물자원종합관리) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2018-보건의료생물자원종합관리) by Research of Korea Centers for Disease Control and Prevention.


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