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Conidiogenesis-related DNA photolyase gene in Beauveria bassiana
  • 작성일2019-05-09
  • 최종수정일2019-05-09
  • 담당부서연구기획과
  • 연락처043-719-8033
  • 1,089
Journal of invertebrate pathology, 2018, 153, 85─91, DOI: https://doi.org/10.1016/j.jip.2018.02.013

Conidiogenesis-related DNA photolyase gene in Beauveria bassiana

Se Jin Lee, Lee MR; Kim S; Kim JC; Park SE; Shin TY; Kim JS

Abstract

    Beauveria bassiana is an entomopathogenic fungi used in environmentally mindful pest management. Its main active ingredient, conidia, is commercially available as a fungal biopesticide. Many studies of conidia production have focused on how to optimize culture conditions for maximum productivity and stability against unfavorable abiotic factors. However, understanding of how conidiogenesis-related genes provide improved conidial production remains unclear. In this study, we focus on identifying conidiogenesis-related genes in B. bassiana ERL1170 using a random mutagenesis technique. Transformation of ERL1170 using restriction enzyme-mediated integration generated one morphologically different transformant, ERL1170-pABeG #163. The transformant was confirmed to represent B. bassiana, and the binary vector was successfully integrated into the genome of ERL1170. Compared to the wild type, transformant #163 showed very slow hyphal growth and within 6 days only produced <1×106 conidia/0.28 cm2 agar block (wild type: 6.2×107 conidia/agar block). Transformant #163 also exhibited different morphology than the wild type, including thicker hyphae with some club-shaped parts. In contrast, the typical morphology of wild type B. bassiana exhibits thread-like hyphae and conidiophore structures and circular conidia. To determine the location of the randomly inserted DNA, we conducted thermal asymmetric interlaced (TAIL) PCR and Escherichia coli cloning to clearly sequence the disrupted region. We identified one colony (colony No. 7) with an insertion site identified as DNA photolyase. This was confirmed through a gene knock-out study. It is possible the gene that encodes for DNA photolyase was disrupted during the insertion process and might be involved in fungal conidiogenesis. This work serves as a platform for exploring the function of a variety of B. bassiana genes involved in pest management and their downstream processing.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2017-ER5501-01) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2017-ER5501-01) by Research of Korea Centers for Disease Control and Prevention.


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