Stem Cell Research, 2017, 01, 46─48, DOI: https://doi.org/10.1016/j.scr.2016.12.028

Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9

So-Jung Kim, Omer Habib; Jin-Soo Kim; Hyo-Won Han; Soo Kyung Koo; Jung-Hyun Kim

Abstract

    Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.



  • 본 연구는 질병관리본부 연구개발과제(과제번호 2017-NG61004-00) 연구비를 지원받아 수행되었습니다.
  • This research was supported by a fund(code 2017-NG61004-00) by Research of Korea Centers for Disease Control and Prevention.


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